o-Phenylenediamine dihydrochloride SIGMA P8412-50TAB

e5a49a91caf2

21,798.05

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**o-Phenylenediamine dihydrochloride SIGMA P8412-50TAB** tablet, 30 mg substrate per tablet, **Reconstitution** * Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). , * Add 40 ?l of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. , * Tablets are individually packaged in foil packets., **Application** * o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. , * The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. , * The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.,

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**o-Phenylenediamine dihydrochloride SIGMA P8412-50TAB** tablet, 30 mg substrate per tablet, **Reconstitution** * Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). , * Add 40 ?l of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. , * Tablets are individually packaged in foil packets., **Application** * o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. , * The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. , * The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.,

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