SensiFAST SYBR Hi-ROX One-Step Kit

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SensiFAST SYBR Hi-ROX One-Step Kit

Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions.

Product Highlights

  • Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
  • Reproducible – consistent results between technical replicates for increased confidence in results
  • Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
  • Robust – reliable detection of RNA targets from a broad range of sample types
  • Fast – delivers reproducible, accurate assay results in as little as 40 minutes

Product Description

The SensiFAST™ SYBR® Hi-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.

An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.

The SensiFAST SYBR® Hi-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX.

Storage & Stability

  • All kit components should be stored at -20°C upon receipt for optimum stability.
  • Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
  • When stored under the recommended conditions and handled correctly.
  • full activity of the kit is retained until the expiry date indicated on the outer box label.


Cat. No.




100 Reactions 12090
BIO-73005 500 Reactions



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